Bilirubin, Total

Useful For

Measurement of the levels of bilirubin, an organic compound formed during the normal and abnormal destruction of red blood cells, is used in the diagnosis and treatment of liver, hemolytic, hematological, and metabolic disorders, including hepatitis and gall bladder blockage.

Method Name

Colorimetric diazo method


Bilirubin, Total; T. Bili, Bilirubin, Total

Specimen Type

Plasma or serum

Specimen Required

Lithium heparin plasma separator tube is preferred but serum separator tube (SST) is also acceptable. If using an SST, please allow 20 minutes after collection for specimen to clot before centrifugation. All specimens must be centrifuged within four hours of collection.

Specimen Minimum Volume

0.5 mL of blood

Specimen Stability Information

If protected from light, specimen is stable for 24 hours refrigerated.

Performing Laboratory

Glens Falls Hospital Chemistry and Special Chemistry Department

Reference Values

Adult: 0.0-1.2 mg/dL Pediatric: See interpretive data

Day(s) and Time(s) Performed

As ordered. Turn around time four hours.

Specimen Retention Time

72 hours