Useful For
Identify and characterize the following:
• Reactive lymphocytosis vs chronic lymphocytic leukemia (CLL)
• CLL vs mantle cell lymphoma
• Prolymphocytic leukemia vs lymphoblastic leukemia large granular lymphocyte proliferations, T-γ lymphoproliferative disease, natural killer cell proliferations, T-cell CLL, T-cell γ/δ proliferations
• Sézary syndrome
• Non-Hodgkin lymphoma
• Adult T-cell leukemia/lymphoma
Neoplastic B-cell proliferations (chronic leukemias and lymphomas) are clonal expansions of cells that express either κ or λ immunoglobulin light chains.
Method Name
Immunophenotyping by flow cytometry
Important Note
These studies require that the tissue to be handled promptly as it is sent to a reference laboratory for additional studies. Please notify the Histology Department prior to sending the specimen as this will help to prevent misplacement, delays, or other adverse events.
- Between 7:30 a.m. and 5:00 p.m. Monday – Friday please contact the Histology Department at 518-926-3832.
- Please note: The cut-off time for receiving flow cytometry specimens is 3:00 p.m. to ensure timely handling and shipping to the reference laboratory.
- Weeknights after 5 p.m. and on weekends, call the main GFH laboratory at 518-926-3800 and ask for the on-call Pathologist.
Aliases
Lymph Node Studies, Surgical Pathology, Histology, Leukemia Immunophenotyping, Lymphoma Immunophenotyping
Specimen Type
Tissue
Specimen Required
Fresh lymph node tissue should be submitted in a dry, sterile container along with a completed request slip.
- For lymph node core biopsies, a small amount of normal saline may be added to keep the tissue from drying out.
- Alternatively, tissue can be submitted in RPMI culture media
- Transport specimen to the Histology Department as soon as possible after collection
Specimen Minimum Volume
- 0.5 to 1.0 cm3 fresh tissue
Specimen Stability Information
- Fresh tissues, tissue in saline, or tissue in RPMI media should be transported immediately to the laboratory Histology Department.
Rejected Due To
- Specimen frozen
- Specimen in formalin or other fixative
- Contaminated transport medium
Performing Laboratory
Tissue specimens for flow cytometry are sent to Integrated Oncology reference laboratory.
- Exception: for patients with BlueShield of Northeastern New York health insurance only, flow cytometry specimens will be sent to Quest Diagnostics reference laboratory.
Day(s) and Time(s) Performed
Monday – Friday beginning at 7:30 a.m. – 3:00p.m.
The cut-off time for receiving flow cytometry specimens is 3:00 p.m. to ensure timely handling and shipping to the reference laboratory.
Analytical Time
Expected turn around time:
Integrated Oncology:
- Flow Cytometry: 2 business days
- Hematopathology Consult: 6-8 business days
Quest Diagnostics:
- Flow Cytometry: 7-8 days
- For expected turn around times for Quest Diagnostics Hematopathology Consult Reports please contact them at this link: Quest Diagnostics
Specimen Retention Time
The specimens are retained by the appropriate reference laboratory. The reference laboratories can be reached at the links below:
CPT Code Information
For CPT code information please contact:
Analytical Time
Expected turn around time:
Integrated Oncology:
- Flow Cytometry: 2 business days
- Hematopathology Consult: 6-8 business days
Quest Diagnostics:
- Flow Cytometry: 7-8 days
- For expected turn around times for Quest Diagnostics Hematopathology Consult Reports please contact them at this link: Quest Diagnostics